Fig. 2
From: Tumor-reprogrammed resident T cells resist radiation to control tumors
Preexistent intratumoral T cells maintain their motility after IR. T cell motility was analyzed in the experiments from Fig. 1. a The motility of irradiated preexisting EYFP+ cells is comparable to newly infiltrating T cells present in the same tumor regions, as evidenced by similar average speeds and arrest coefficients at any time point analyzed. Data are from 20 to 25 min movies and 21 to 35 μm deep optical sections (Z-stack). Comparisons for average speed and arrest coefficient between preexistent and new T cells were non-significant at all time points post-IR (unpaired t-test). The number (N) of cell tracks quantified was as follows (day after beginning of IR; N preexistent T cells, N new T cells): 20 Gy mouse (d0; 204, 10–d1; 216, 18–d2; 184, 40–d3; 114, 33), 1.8 Gy × 5 mouse (d0; 471, 56–d1; 238, 62–d2; 149, 51–d4; 232, 53), Non-IR (d0; 206, 36–d2; 207, 86–d4; 254,104). b, d Tracks followed by preexistent (yellow) and new (green) T cells within the same tumor region 3 days after IR. c, e Spider plots show separately preexistent and new T cell tracks, from b and d, respectively. Scale bars: 50 μm
