Fig. 5
From: Tumor-reprogrammed resident T cells resist radiation to control tumors
Intratumoral T cells are transcriptionally similar to TRM and are more sensitive to IR in mice treated with anti-TGFβ antibodies. Gene expression data from LN vs. tumor T cells (Fig. 4) was compared to published data on gene expression differences between TRM cells and spleen memory T cells (GEO GSE47045) to estimate similarity across all samples. a PCA-style plot shows that tumor T cells were phenotypically closest to skin and other TRM, whereas LN T cells were closest to spleen naïve or memory T cells. b Eighteen of 37 genes from the published TRM signature were differentially expressed also in intratumoral T cells. c Total CD3+ T cells were quantified 24 h after 8 Gy WBI of mice bearing established MC38 tumors that had been treated or not with anti-TGFβ blocking antibodies every 2–3 days starting 2 days after tumor cell inoculation until day of sacrifice. Data are pooled from two independent experiments with a total of nine mice per group. The plot shows mean and SD. n.s., non-significant; *P = 0.01 (unpaired t-test). Each dot represents an individual mouse. d The effect of IR in mice from c was calculated as percent decrease in the average number of T cells per gram of tumor. The percent decrease in the average number of T cells per microliter of blood in a subset of mice from c that were bled before sacrifice (N = 3–4 per condition) is shown as a reference
