Fig. 3
Il36rn−/− mice are protected from diet-induced obesity and insulin resistance. a–h Male Il36rn−/− and wt mice of the same age were fed a HFD (60 kcal% fat) for 8–10 weeks starting from 8 weeks of age. Weights (a) and daily food intake per mouse (b) were monitored and data shown are pooled from three independent experiments (wt n = 15, Il36rn−/− n = 22). c EAT and SAT depots mass was measured after 10 weeks in HFD. d, e Intraperitoneal GTT on wt and Il36rn−/− mice after 8 weeks in HFD; glucose over time after i.p. glucose injection (d) and AUC (e) shown. f, g Intraperitoneal ITT on wt and Il36rn−/− mice after 9 weeks in HFD; glucose over time after insulin bolus-injected i.p. (f) and AUC (g) shown. h Fasting blood insulin levels for wt and Il36rn−/− mice after 10 weeks in HFD. i Relative protein expression levels of phospho-Akt (P-Akt)/total Akt (T-Akt) and phospho-insulin receptor β (P-IR)/total insulin receptor β (T-IR) in the liver, skeletal muscle and EAT of wt (n = 7) and Il36rn−/− (n = 4) mice after insulin injection, as indicated by densitometry analysis of western blot data (in Source Data file). j–l Protein expression of P-IR, T-IR, P-Akt and T-Akt in the liver (j), skeletal muscle (k) and EAT (l) of representative wt (n = 3) and Il36rn−/− (n = 3) mice after insulin injection. Data represent means ± SEM. Statistical analysis using two-tailed unpaired student’s t-test for (c), (g) and (i), two-tailed Mann–Whitney test for (b), (h) and (e) and RM two-way ANOVA for (a), (d) and (f). ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file
