Fig. 7
Microbiome analysis and obesity phenotype of cohoused wt and Il36rn−/− mice. a Weighted ordination analysis of beta-diversity of intestinal microbiome composition in cohoused for 8 weeks and separately housed wt and Il36rn−/− mice (n = 5 per group). b Proportional relative abundance of the most abundant phyla in the microbiome of cohoused wt and Il36rn−/− mice. c Percent relative abundance means ± SD of the eight most abundant phyla (including unclassified phylum), Kruskal–Wallis rank sum test to determine significance. d Percent relative abundance of A. muciniphila in regard to total bacteria before and after cohousing wt and Il36rn−/− mice for 4 and 8 weeks, as determined by qPCR (n = 4 per group). e–h Aged matched male wt and Il36rn−/− mice were cohoused since weaning in the same cage for 8 weeks (n = 4 per group). They were then given HFD for 8–10 weeks, and weights were monitored weekly (e). f EAT and SAT depots mass after 10 weeks in HFD. g, h GTT on cohoused mice after 8 weeks in HFD, glucose over time (g) and AUC (h) shown. i–j ITT on cohoused mice after 9 weeks in HFD, glucose over time (i) and inverted AUC (j) shown. Data show means ± SEM unless otherwise described. Statistical analysis using two-tailed Mann–Whitney test for d, two-tailed unpaired student’s t-test for (f), (h) and (j), RM two-way ANOVA for (e), (g) and (i) or as described. ns p > 0.05, *p < 0.05, **p < 0.01. Source data are provided as a Source Data file
