Fig. 5
Experimental validation of GPR151 and PDE3B function in adipogenesis. a, b qPCR analysis of gene expression patterns of PDE3B and GPR151 during (a) mouse 3T3-L1 adipogenesis and (b) human SGBS adipogenesis. For 3T3-L1 cells, n = 3 independent samples for day 0, n = 4 for day 10. For SGBS cells, n = 3 independent samples for all timepoints of PDE3B analysis, n = 5 for all timepoints of GPR151 analysis. c qPCR analysis of Gpr151 mRNA knockdown in 3T3-L1 preadipocytes, by 3 siRNAs targeting Gpr151 individually and together. n = 3 independent experiments. d qPCR analysis of the effect of siGpr151 knockdown (individually and together) on adipogenesis markers, Pparg, Cebpa and Fabp4. n = 3 independent experiments. e–g Oil-Red O staining (e), quantification of lipid droplets (f), and lipolysis (g) in scRNA- or siGpr151-tansfected adipocytes. ×10 magnification. Scale bar = 100 μm. For ORO imaging and quantification, n = 10 independent cell cultures for scRNA, n = 12 for siGpr151. For lipolysis, n = 3 independent experiments. Means ± SEM are shown (***p-value < 0.001, **p-value < 0.01, *p-value < 0.05). scRNA: scrambled siRNA. ISO: isoproterenol. Source data are provided as a Source Data file
