Fig. 7
RASP2 contains lipid binding domains necessary for rhoptry secretion. a Schematic representation of TgRASP2 domains and the TgRASP2con2 recombinant expression construct. b Immunoblots of recombinant TgRASP2con2 and TgRASP2con2MUT associated or not with liposomes containing phosphatidylethanolamine (PE) and phosphatidylcholine (PC) (proportions 10%: 90%) phosphatidic acid (PA), PE and PC (proportions 30%: 10%: 60%) and phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), PE and PC (proportions 25%: 10%: 65%). Ctrl, no lipid control; SN, supernatant fraction (unbound); P, pellet fraction (bound protein); Immunoblots were probed with mouse anti-His antibodies. Blots are representative of ≥ three independent experiments. c Quantification of liposome binding assays. p values generated from Student’s t test. Error bars represent ±SD from three or more replicates. d Rhoptry secretion assay with Cpt_WT, ∆PH, L3* and the double mutant ∆PH_L3*. One representative experiment out of three independent assays. Source data are provided as a Source Data file
