Fig. 4

Translational coupling via the TeRe mechanism in H. volcanii and E. coli. a Schematic overview of the constructs for H. volcanii. The native overlapping gene pairs are shown in magenta and blue, the dhfr reporter gene is shown in green. The intragenic SD motif is indicated by a yellow bar. The designed premature stop codon is indicated. b. Normalized translational efficiencies of the native gene pairs lacking a premature stop codon (-) and the stop codon variants (+). The names of the gene pairs are shown at the bottom. Average results of three biological replicates and their standard deviations are shown. Source data are provided as Source Data file. c Schematic over view of the constructs for H. volcanii. The native upstream gene is shown in magenta, the dhfr reporter gene is shown in green. The intragenic SD motif is indicated by a yellow bar. The designed premature stop codon is indicated. d Normalized translational efficiencies of the native genes lacking a premature stop codon (−) and the stop codon variants (+). The names of the genes are shown at the bottom. Average results of three biological replicates and their standard deviations are shown. Source data are provided as Source Data file. e Schematic overview of the constructs for E. coli. The native overlapping gene pairs are shown in magenta and blue, the glpD reporter gene is shown in purple, the dhfr reporter gene is shown in green. The intragenic SD motif is indicated by a yellow bar. The presence of a stop codon is indicated. f Normalized glpD translational efficiencies of the native gene pairs lacking a stop codon (−) and the stop codon variants (+). The names of the gene pairs are shown at the bottom. Average results of three biological replicates and their standard deviations are shown. Source data are provided as Source Data file. g Normalized gusA translational efficiencies of the native gene pairs lacking a stop codon (−) and the stop codon variants (+). The names of the gene pairs are shown at the bottom. Average results of three biological replicates and their standard deviations are shown. Source data are provided as Source Data file