Fig. 4
PIN1 promotes p-TRIM59S308 association with importin α5. a Schematics of TRIM59 WT and nuclear localization sequence (NLS) mutant (NLS-mut). b Representative images of TRIM59 nuclear translocation in LN229/EGFR cells resconstituted with TRIM59 WT or NLS mutant. Scale bar, 40 μm. c WB for effect of TRIM59 WT or NLS-mut on TRIM59 nuclear translocation. d IP and WB for TRIM59 binding to importin α proteins indicated. Flag-tagged importin α proteins and HA-tagged TRIM59 were co-expressed in LN229/EGFR cells. e Effect of importin α5 knockdown on TRIM59 nuclear translocation. f Effects of TRIM59 WT, S308A, or NLS-mut expression on TRIM59 binding with importin α5. Flag-tagged importin α5 was transfected into LN229/EGFR cells with HA-TRIM59 WT, S308A, NLS-mut mutant, or an empty vector control. g WB for effects of ectopic expression of PIN1 WT or C113A mutant on TRIM59 binding with importin α5. In b–f, various cells were stimulated with or without EGF (100 ng/ml) for 6 h. Data are representative of three independent experiments with similar results. Source data are provided as a Source Data file
