Fig. 5 | Nature Communications

Fig. 5

From: CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity

Fig. 5

Nuclear TRIM59 associates with macroH2A1. a Silver staining showing the nuclear TRIM59-associated proteins in LN229/EGFR cells with EGF stimulation. b IP and WB for TRIM59 binding with macroH2A1.1 (mH2A1.1) and macroH2A1.2 (mH2A1.2) proteins. c Representative images of co-localization of TRIM59 with mH2A1. Scale bar, 40 μm. d WB for effects of TRIM59 S308A and NLS-mut on TRIM59 binding with mH2A1 in U87/EGFR/shTRIM59 cells. In b–d, various cells were stimulated with or without EGF (100 ng/ml) for 6 h. Data are representative of three independent experiments. Source data are provided as a Source Data file

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