Fig. 6

Scute acts downstream of Klu in enteroendocrine differentiation. a, b Control esg-F/O clones 14 days after clonal induction. c, d Expression of klu^RNAi lead to increased EE differentiation in clones, marked by increased numbers of Pros⁺-cells (red) (c, d, quantification in i, j). e, f sc^RNAi clones showed almost no EE differentiation. g, h Similarly, the combination of klu^RNAi with sc^RNAi resulted in clones lacking EE differentiation. Representative areas of posterior midgut are shown. i Quantification of GFP⁺/Pros⁺ double-positive cells/clone of the genotypes in a–h. n = 10 for control, n = 14 for klu^RNAi, n = 10 for sc^RNAi and n = 12 for sc^RNAi;klu^RNAi. j Quantification of GFP⁺/Pros⁺ double-positive cells/clone of esg-F/O clones expressing either UAS-sc, UAS-klu, or the combination. See Supplementary Fig. 7 for images. n = 5 for control, n = 6 for UAS-klu, UAS-sc, and UAS-klu;UAS-sc. Error bars represent mean ± SD. Significance was calculated using Student’s t-test with Welch’s correction. Scale bar = 50 µm