Fig. 3
Tandem repeats of HJURP R1 or R2 rescue the localization of HJURP and CENP-A deposition. a Representative images showing the fluorescence of SNAP-CENP-A and EGFP-HJURP variants in fixed HeLa cells treated as described in Fig. 2a. White scale bars indicate 10 µm. b, c Quantification of the centromere fluorescence intensity of SNAP-CENP-A or EGFP-HJURP variants. The bar graphs represent mean values from three replicate experiments (blue dots indicate the mean values from each experiment). Error bars indicate standard deviations. d SDS-PAGE results of amylose-resin pull-down assays showing the increase of HJURP binding to Mis18core when tandem repeats of R1 and R2 were used. The numbers in the parenthesis indicate the concentrations (µM) of the proteins used in the assays. Source data are provided as a Source Data file
