Fig. 2
ORF2p is crucial for intestinal infection by EV-A71. a–c One-step growth curve of EV-A71 and EV-A71ΔORF2p in RD, HT-29 and isolated primary IECs at an MOI of 10. Error bars denote SEM; ANOVA test, n = 3 biologically independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001. d HT-29 cells stably expressing EV-A71 ORF2p or control HT-29-CDH cells generated by transduction with the empty lentiviral vector pCDH-CMV-MCS-EF1-Puro were challenged with EV-A71 WT or EV-A71ΔORF2p at an MOI of 10. Viral titres were determined at 0, 3, 6, 9, and 12 hpi (n = 3 biologically independent experiments). Error bars denote SEM. e HT-29 cells expressing the ORF2p protein derived from EV-A71 sub-genotypes A, B0, B1, B2, B3, B4, B5, C1, C2, C3, C4 and C5 were infected with EV-A71ΔORF2p at an MOI of 0.1. Viral titres were determined at 72 hpi (n = 3 biologically independent experiments). Error bars denote SEM. Source data are provided as a Source Data file
