Fig. 6 | Nature Communications

Fig. 6

From: Proton-dynamic therapy following photosensitiser activation by accelerated protons demonstrated through fluorescence and singlet oxygen production

Fig. 6

Proton-beam induced singlet oxygen and fluorescence degradation. Singlet oxygen phosphorescence, registered during proton beam irradiation at a fluence rate of 3.2 × 109protons cm−2 sec−1 in acetone d6 solutions of a Erythrosin B (ErB) (60 μM) and b Rose Bengal (RoseB) (50 μM). c Protoporphyrin IX (PpIX) photoproduct (photoprotoporphyrin) production in the presence and absence of the singlet oxygen-specific scavenger 1,4-diazabicyclo[2.2.2]octane (DABCO) (200 mM) following proton irradiation of 50 μM PpIX in dimethyl sulfoxide (DMSO). The spectra were taken with a spectrofluorometer at 405 nm excitation. d Time evolution of PpIX (50 μM, DMSO) fluorescence intensity at 635 nm against irradiation dose either by a proton beam (crossed symbols) or by light (Xenon lamp, 350–750 nm, open symbols), in the presence (red squares) or absence (black circles) of DABCO (200 mM). In both cases, data were normalised to the unirradiated 635 nm maximum. The fluorescence data of panels c and d were acquired at 405 nm excitation

Back to article page