Fig. 3
Cellular and molecular determinants of DCS resistance in M. tuberculosis. a qPCR results for differential gene expression (log2 transformed) of selected genes in the DCSR mutants grown in the absence or presence of DCS (10 × MIC for 4 h). The results are representative of three biological replicates, n = 3. b Western blot analysis of Alr expression in the DCSR mutants and parent strain grown in the presence or absence of DCS (10 × MIC for 4 h). HisG levels were used as loading control. The data are representative of two independent experiments. c Illustration of the active site of Alr from M. tuberculosis, 1XFC [https://www.rcsb.org/structure/1XFC] highlighting the location of residue D322 and its interactions with the pyridoxal 5’-phosphate cofactor through a water molecule-hydrogen bond network. d Reaction catalyzed by Alr and its inhibition by DCS. e pH-rate profile of the reaction catalyzed by wild-type and D322’N Alr. f, g Inactivation kinetics of wild-type (WT) and D322’N Alr by DCS at pH 7.5. Source data are provided as a Source Data file
