Fig. 2
Optogenetic stimulation of somatosensory flow to the DLS. a Schematic representation of experimental configuration and anatomical confirmation of infections. b Spike rasters (top) and average peri-event histograms (bottom) of three illustrative units aligned to the onset of optical stimulation of the VPL and recordings in S1(left) or DLS (middle), and simultaneous stimulation and recording in the DLS (right). c Averaged firing rates for cells recorded in S1 (top) and DLS (middle) after stimulation of the VPL or its terminals in the DLS (bottom), expressed as Z-score (color coded) and sorted according to the time they reached the highest activity after stimulus onset. Histograms of the response latencies for all cells recorded in S1 (d) and DLS (e); insets display latencies shorter than 60 ms. For comparison, the distribution of latencies evoked by somatic stimulation is presented in light gray. Box plots indicate median and 25th and 75th percentiles of the latencies evoked by somatic stimulation or stimulation of the VPL or its terminals in S1 (d) or DLS (e)
