Fig. 3 | Nature Communications

Fig. 3

From: Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle

Fig. 3

Characterization of ConM, ZM197M, and AMC011 SOSIP-I53-50NP assembly. a SEC chromatograms of assembled ConM, ZM197, and AMC011 SOSIP-I53-50NPs (from top to bottom). b Raw NS-EM micrographs and 2D-class averages of the pooled SOSIP-I53-50NP fractions corresponding to the SEC chromatogram to the direct left. Particles were subjected to a freeze–thaw cycle prior to NS-EM analysis. The AMC011 SOSIP-I53-50NP solution was mixed 3:1 with Protein Stabilizing Cocktail (ThermoScientific) prior to freezing. The white bar corresponds to 200 nm. Source data are provided as a Source Data file

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