Table 2 Cryo-EM data collection, refinement, and validation statistics
BG505 SOSIP-I53-50NP (EMDB-20261) (PDB 6P6F) | |
|---|---|
Data collection and processing | |
Magnification | 38,168 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 46.6 |
Defocus range (μm) | 1.2–5.1 |
Pixel size (Å) | 1.31 |
Symmetry imposed | I1 |
Initial particle images (no.) | 3996 |
Final particle images (no.) | 3590 |
Map resolution (Å) | 4.5 (0.143) |
FSC threshold | |
Map resolution range (Å) | 4.4–6 |
Refinement | |
Initial model used (PDB code) | NA |
Model resolution (Å) | NA |
FSC threshold | |
Model resolution range (Å) | NA |
Map sharpening B factor (Å2) | −149 |
Model composition | |
Non-hydrogen atoms | 0 |
Protein residues | 351 |
Ligands | 0 |
B factors (Å2) | NA |
Protein | |
Ligand | |
R.m.s. deviations | |
Bond lengths (Å) | 0.017 |
Bond angles (°) | 1.586 |
Validation | |
MolProbity score | 0.5 |
Clashscore | 0 |
Poor rotamers (%) | 0 |
EMRinger score | 1.55 |
Ramachandran plot | |
Favored (%) | 99.7 |
Allowed (%) | 0 |
Disallowed (%) | 0 |
