Fig. 7
From: Heme and hemoglobin utilization by Mycobacterium tuberculosis
M. tuberculosis survival in macrophages depends on heme and iron utilization. Growth of Mtb H37Rv (wt; circles), the Δdpp mutant ML2437 (triangles), and the complemented Δdpp mutant (squares) in HdB minimal medium with a 10 µM ammonium ferric citrate and b 2.5 µM human hemoglobin (cyan) or 10 µM hemin (blue). c THP-1 cells were differentiated with phorbol myristate acetate (PMA) and infected at an MOI of 10 with wt Mtb H37Rv (black) and the ΔmbtD (orange) and Δdpp (cyan) mutant strains. Survival of the Mtb strains in macrophages was determined by plating on agar plates and counting colony-forming units (CFU). Asterisks denote significant differences as determined by Tukey’s HSD following an F-test (p < 0.05) compared to wt. n.s.: not significant. Error bars represent standard errors of mean values of biological triplicates. Source data are provided in the Source Data file
