Fig. 6
bes1-D and bzr1-1D can partially suppress anther developmental defects and male sterility of tpd1. a–e Toluidine blue stained anther semithin sections of Col-0 (a), tpd1 (b), tpd1 bes1-D (c), tpd1 bzr1-1D (d), and tpd1 bes1-D bzr1-1D (e). f–j Alexander stained anthers of Col-0 (f), tpd1 (g), tpd1 bes1-D (h), tpd1 bzr1-1D (i), and tpd1 bes1-D bzr1-1D (j). k–o Inflorescences of Col-0 (k), tpd1 (l), tpd1 bes1-D (m), tpd1 bzr1-1D (n), and tpd1 bes1-D bzr1-1D (o). Tapetum layer (T), and microsporocytes (Ms) are indicated by arrows. p Quantitative RT-PCR analyses to show relative gene expression levels of tapetum marker genes, A6, A9, ATA7, and DYT1 in the inflorescences of Col-0, tpd1, tpd1 bes1-D, tpd1 bzr1-1D, and tpd1 bes1-D bzr1-1D. Data are presented as mean and s.d. (n = 3). Statistically significant differences between groups were tested using one-way ANOVA followed by LSD (least significant difference) post hoc test. Different letters indicate significant difference at P < 0.05. Scale bars represent 20 μm in (a–e), 50 μm in (f–j), and 1 cm in (k–o)
