Fig. 2
From: A cell fitness selection model for neuronal survival during development
Regulation of TRKC levels in PSNs and their correlation with differential survival. a Quantification of Ntrk3 in E11.5 DRG cultured for 6 h with or without NT3 (50 ng/ml) (n = 4). b TRKC/RUNX3 immunostaining on E12.5 DRG sections from Bax−/− and Bax−/−;NT3−/− animals34. Scale bar: 50 μm. c Quantification of TRKC levels in individual PSNs from E12.5 Bax−/− and Bax−/−;NT3−/− animals in b (n = 2), with similar number of PSNs in both Bax−/− and Bax−/−;NT3−/− DRG (P = 0.83, unpaired Student’s t-test). d Correlation between RUNX3 and TRKC levels in individual E11.5 PSNs. e TRKC expression in E11.5 DRG sections from Runx3+/+ and Runx3−/− animals. Scale bar: 20 μm. f Quantification of TRKC intensity in individual PSNs in E11.5 Runx3+/+ and Runx3−/− animals. **P < 0.01. Bar graph: number of TRKC+ neurons in E11.5 Runx3+/+ and Runx3−/− animals; ns, not significant, unpaired Student’s t-test (n = 3). g Immunostaining for TRKC, RUNX3 and p-AKT on E11.5 DRG sections and quantified correlation between TRKC and p-AKT levels in individual PSNs (right panel). h Survival of E11.5 DRG neurons from Runx3+/+ and Runx3−/− animals treated with NT3 for 48 h (*P < 0.05, n = 4–6, one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test). i Scheme representing the in ovo electroporation (HHst 13/14, or E2). j Cross section of electroporated HHst26/27 embryos (E5) showing transfected cells in half spinal cord (SC) and in the ipsilateral DRG. k Quantification at E5 (before cell death period) of TRKC intensity among transfected PSNs (RUNX3+/TRKC+). ****P < 0.0001, unpaired Student’s t-test. l Comparison before (E5) and after cell death period (E7) of the % of PSNs in transfected GFP+ neurons. **P < 0.01, unpaired Student’s t-test (n = 4). m TRKC and RUNX3 expression in PSNs of E13.5 Bax+/+ and Bax−/− DRG sections. (Right) Boxed regions (1) and (2) are magnified. Arrowheads show PSNs with lower size (see Supplementary Fig. 6c). Scale bar: 50 μm. n Quantification of TRKC levels in PSNs from E13.5 Bax+/+ and Bax−/− DRG neurons (n = 4). Dashed line distinguishes TRKCHigh and TRKCLow populations. Source data are available as a Source Data file
