Fig. 4
Functional relevance of p63 in myoepithelial cells. a Xenograft tumor weight of shTP63-expressing MCF10DCIS cells with or without doxycycline following mammary fat pad, intraductal, or subcutaneous injection. p-value indicates statistical significance of difference in tumor weight between groups based on t-test. Mean ± SD shown. b Hematoxylin–eosin (H&E) staining and immunofluorescence analysis of SMA and p63 expression. Scale bar 100 μm. c Pathway enrichment analysis of genes up-regulated or down-regulated following shTP63 expression in MCF10DCIS cells. Color scale corresponds to −log(p-value) of significance of enrichment, calculated by MetaCore enrichment analysis test. d–f Immunoblot analysis of p63 expression levels in MCF10DCIS cells following detachment from matrix d, detachment and concomitant treatment with MG132 (10 µM), Staurosporin (STS, 10 µM), and Bafilomycin A1 (BAF 100 nM) e, and treatment with inhibitors of various signaling pathways [Rapamycin (mTOR), Y15 (FAK) 5 µM; Sonidegib (Hh), XAV393 (WNT), LY2157299 (TGFβ), Verteporfin (YAP), PD0325901 (MEK) 10 µM; Dasatinib (SRC) 2 µM] in adherent conditions f. GAPDH serves as loading control. g Heatmap depicting unique and H3K27ac overlapping p63 peaks. The color key is the score of ChIP-seq signal over selected genomic region, the signals across different genomic regions have scaled to the same length. h Hockey stick plot depicting super-enhancers in MCF10DCIS cells. i Predicted protein interaction network of TFs identified as core transcriptional regulatory circuits in MCF10DCIS cells. Legend indicates the source of data used to determine interactions. TFs that are not part of the network were removed. j Integration of differential gene expression and p63 targets by BETA analysis. The p-value listed in the top left represents the significance of the UP or DOWN group relative to the NON group as determined by the Kolmogorov–Smirnov test. Source data are provided as a Source Data file
