Fig. 6
Energetic costs between migration choices govern migration decision-making. a Model predictions for migration choice as a function of the difference in energetic costs between migration paths with increasing cell stiffness. b ΔATP:ADP for migration into the 12 and 7 μm microtrack following pharmacological treatments and siCav1 knockdown (n = Ctrl: 91, 118; Rho+: 92, 107; CL-A: 95, 100; Y27: 117, 135; ML7: 95, 107; MβCD: 105, 123; siCtrl: 49, 53; siCav1: 46, 55 cells for 12 μm and 7 μm microtracks). c Percentage of migration into the 7 μm microtracks as a function of ΔATP:ADP 7–12 with changes in cell stiffness; dashed line shows one-phase decay. d The inverse relationship between migration frequency into the 7 μm microtracks and ΔATP:ADP 7–12 as a function of cell stiffness across treatments; dashed line shows one-phase decay for migration into 7 μm and one-phase association for ΔATP:ADP 7–12. e Model predictions for migration choice as function of energetic cost with increasing matrix stiffness. f ΔATP:ADP between migration paths with changing matrix stiffness (n = 0 mM: 30, 31; 100 mM: 31, 29 cells for 12 μm and 7 μm microtracks). g Percentage of migration into the 7 μm microtracks as a function of ΔATP:ADP 7–12 with changes in matrix stiffness. h The inverse relationship between migration frequency into the 7 μm microtracks and ΔATP:ADP 7–12 as a function of matrix stiffness. Data shown as median ± interquartile range (box), 5th–95th percentiles (whiskers), and mean (+) (b, f), or mean ± s.e.m. (c, d, g, h); two-tailed Mann–Whitney test (b, f); *P < 0.05
