Fig. 6
Genes involved in insecticide resistance revealed by genome data of the codling moth, Cydia pomonella. SNPs in 667 genes involved in the azinphos methyl (a) and deltamethrin (b) resistance represented by Manhattan plot. The vertical axis shows the P-value and the horizontal axis indicates the Z, W, and 27 autosomes. The red horizontal line represents the genome-wide significance threshold (P = 1 × 10−4). c The SeqLogo plots show the frequency of diploid genotypes, which were significantly different between resistant strains (Raz, resistance to azinphos methyl; and Rv, resistance to deltamethrin) and the susceptible strain (S); the primer pairs used are given in Supplementary Table 25. Ten individuals of each strain were used for the confirmation. d qPCR analysis of the expression level of CYP6B2 (ID: CPOM05212) in Raz and Rv insecticide-resistant strains in comparison with the insecticide susceptible strain S. The transcript abundance of CYP6B2 was 241. Fourfold higher in Raz strain and 77.3-fold higher in Rv strain compared with that of the sensitive strain S. * indicates significant influences (Student’s t test, Rv: P = 0.0102, Raz: P = 0.0002). This gene was constitutively highly expressed in two resistant strains. Error bars indicate + SEM. e The expression levels of the CYP6B2 were significantly reduced after siRNA injection. * indicates significant influences (Student’s t test, Azinphos-methyl, WT: P = 0.0135, siGFP: P = 0.0318). WT indicates individuals without any injection. Error bars indicate + SEM. f The survival rate of C. pomonella fourth instar larvae treated with LC50 concentrations of azinphos methyl (103.50 mg/L), deltamethrin (3.55 mg/L), and imidacloprid (35.35 mg/L) after injection of siRNA. * indicates significant influences (Student’s t test, Azinphos-methyl, WT: P = 0.0132, siGFP: P = 0.0232; Deltamethrin, WT: P = 0.0334, siGFP: P = 0.0409 (one-tail)). Thirty individuals were used for each treatment. Error bars indicate + SEM. Source data are provided as a Source Data file
