Fig. 5
From: Disposable ultrasound-sensing chronic cranial window by soft nanoimprinting lithography
Longitudinal cortical PAM through the usCCW. a Photograph of a free-moving mouse in the breeding cage after the implantation. b Photograph of the mouse mounted under the microscope during the imaging. c Maginfied optical image shows the implanted transparent usCCW and the clear optical view of the cerebral cortax. d Q-factor shows marginal reduction from 4.2 × 104 to 3.6 × 104 over the 28-day-period. Error bars show the standard deviation of measured results. e Maximum amplitute projections of PAM images show cortical vasculature in the same area over a 28-day-period. Medium post-surgical bleeding occurs at Day 3 and the hemorrhage is gradually cleared in 4 days. Aggressive neovasularization is also clearly observed. The pairwised comparison of the measured cortical vasculature between (f) Day 0 and Day 2, (g) Day 0 and Day 14, and (h) Day 14 and Day 28. Superposition of two images rendered in distinct megenta and green colors is used to provide direct visualization of the neovascularization process. The overlapping regions are therefore rendered in white color
