Fig. 2

AxLN-micro-injected tumors metastasize efficiently to distant tissues. a Schematic depicting the injection and expected outcomes of GFP/fL- or mCh/rL-labeled 4T1 cells into the MFP or AxLNs, respectively, of the same mouse. b Representative IVIS imaging of GFP/fL and mCh/rL cells post injection in the same mouse. c Caliper measurements of tumor growth for MFP and AxLN tumors (n = 7 mice). d Fluorescence imaging of post-injected AxLNs after 6 weeks of growth show minimal GFP+ cells. e Flow cytometry quantification of lungs and brains to determine relative metastatic capability of tumors simultaneously established in MFPs and AxLNs. f Ex vivo luciferase signal observed in lungs and brains of mice simultaneously bearing GFP/fL MFP tumors and mCh/rL AxLN tumors. Statistical significance was measured by Mann–Whitney t tests; **p < 0.01