Fig. 3
From: Histone deacetylase 11 inhibition promotes breast cancer metastasis from lymph nodes
LN metastases up- or down-regulate cell cycle-associated genes in a plastic manner. a Pairwise gene expression array comparison between MFP- and AxLN-implanted tumors. b Pairwise gene expression array comparison between AxLN-implanted and AxLN-derived lung metastases. c Venn diagram showing shared genes between the analysis of a, b. d Six possible patterns of gene expression across the three experimental conditions (mammary fat pad, MFP; axillary LN, AxLN; lung metastasis derived from AxLN micro-injection, AxLN-LuM) shown in a, b. e Composite microarray results for the 152 genes that are up- or down-regulated in both pairwise tissue comparisons (cases 2 and 4 of d). Full 206 gene array shown in Supplementary Fig. 4. f Gene ontology analysis (biological processes) of the 152 genes displayed in e. g Cell growth assay for the ex vivo clones represented in the microarray analysis. Statistical significance was measured by unpaired t tests. h Average expression values (RT-qPCR) for several target genes revealed by the microarray analysis. All statistical comparisons are to the axillary LN tumor samples. Statistical significance was measured by ANOVA. i Relative expression of HDAC11 between cell lines derived from MFP tumors and matched de novo AxLN metastasis vs. micro-injected AxLNs obtained 1 or 2 weeks post injection. j Bisulfite sequencing of MFP, AxLN, and AxLN-LuM sub-clones at the HDAC11 promoter CpG island. Statistical significance was measured by unpaired t tests; p values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001
