Fig. 6
Immune effect and in vivo toxin neutralization of nanoreactors. a The scheme demonstrating that nanoreactors stimulate the body’s immune response and improve the therapeutic effect of bacterially infected mice. b Flow cytometric analysis of cells in the draining lymph node at 21 days post administration with RFP-CaO2@PCM@Lec nanoreactors, toxin, heated toxin, or nano-toxin (n = 3; mean ± SD). Cells were first gated on the B220 + IgD low population and values are expressed as percentage GL-7+. c Multivalent antibody responses in vivo. Mice were vaccinated with RFP-CaO2@PCM@Lec nanoreactors, toxin, heated toxin, or nano-toxin on day 0 with boosts on day 7 and 14. On day 21 post first vaccination, the serum was sampled and analyzed for the presence of IgG antibody titers against toxin. d Representative images demonstrating the varying degrees of hemolysis. e Comparison of hemolysis induced by antibody generated by injection of PBS, RFP-CaO2@PCM@Lec nanoreactors, toxin, heated toxin and nano-toxin (n = 3; mean ± SD). f Survival rates of mice over 15 days following an intravenous injection of α-toxin (120 μg kg−1). The mean value was calculated by the t test (mean ± SD). *p < 0.05, **p < 0.01, ***p < 0.001, compared with the indicated group. Source data are provided as a Source Data file
