Fig. 1

HGF-induced EMT in NBT-II carcinoma cells. a Phase contrast images of NBT-II cells at 0, 2, 4, 6, 9 and 24 h post HGF induction. Scale bars: 50 μm. b Cell tracks of NBT-II cells at 0, 2, 4, 6, 9 and 24 h post HGF induction. Data shown are representative of three independent and reproducible experiments. c Bar charts display velocity (left panel), total distance (middle panel) and displacement (right panel) of NBT-II cells over 24 h. Bars represent mean ± SD of three independent experiments. A two-tailed Student’s t test compares the treated versus untreated cell populations, ***P < 0.001. d Representative immunofluorescence images of NBT-II stained with E-cadherin and Vimentin at 0, 4 and 24 h post HGF. Scale bars: 50 μm