Fig. 1
Replication stress induces mitotic arrest and mitotic death. a Time course of live cell imaging throughout this study unless indicated otherwise. b Representative images depicting mitotic outcome and duration from DIC live cell imaging experiments. Time is shown as (hours: minutes) relative to the first image of the series. Scale bar represents 10 µm. c Mitotic duration of IMR90 or IMR90 E6E7 cells following treatment with Dimethyl sulfoxide (DMSO) vehicle, Aphidicolin (APH), or Hydroxyurea (HU) (three biological replicates scoring n ≥ 46 mitoses per condition for IMR90 or n ≥ 66 mitoses per condition for IMR90 E6E7 are compiled in a Tukey box plot, Mann–Whitney test). d Outcome of the mitotic events in (c) (mean ± s.e.m, n = 3 biological replicates, Fisher’s Exact Test). e, f Two-dimensional dot plots for IMR90 (e) and IMR90 E6E7 (f) of mitotic duration and outcome for the data shown in (c, d). Each symbol represents an individual mitosis. T = 0 h is when DMSO or APH were added to the culture. Location of a symbol on the x-axis indicates the time after treatment when that mitosis initiated, the height on the y-axis represents mitotic duration, and the symbol corresponds to mitotic outcome. g Mitotic duration of HT1080 6TG cells following treatment with DMSO, APH, or HU (three biological replicates scoring n ≥ 92 mitotic events per condition compiled in a Tukey box plot, Mann–Whitney test). h Outcome of the mitotic events in (g) (mean ± s.e.m, n = 3 biological replicates, Fisher’s Exact Test). i Two-dimensional dot plots of mitotic duration and outcome from (g, h). j Mitotic duration of HT1080 6TG cultures treated with DMSO, APH or HU ± reversine (REV) (three biological replicates scoring ≥ 112 mitotic events per each condition compiled in a Tukey box plot, Mann–Whitney test). k Outcome of mitotic events in (j) (mean ± s.e.m., n = 3 biological replicates, Fisher’s Exact Test). l Two-dimensional plots of mitotic duration and outcome from (j, k). For all panels, *p < 0.05, **p < 0.01. Source data are provided as a Source Data file
