Fig. 3
Replication stress induces distinct types of mitotic death. a Mitotic duration of HeLa parental and BAX BAK DKO cells following treatment with DMSO or APH (three biological replicates using independent clones scoring n ≥ 32 mitotic events per condition are compiled in a Tukey box plot, Mann–Whitney test, **p < 0.01, ns = not significant). b Two-dimensional dot plots of mitotic duration and outcome in 0.75 µM APH treated HeLa parental and BAX BAK DKO cells from (a). The dashed line identifies the longest duration mitosis observed in the parental cells. c Outcome of the mitotic events in (a) (mean ± s.e.m., n = 3 biological replicates using independent clones, Fisher’s Exact Test, **p < 0.01, ns = not significant). d Representative images of Type 1 and Type 2 mitotic death in 1 µM APH treated HT1080 6TG H2B-mCherry cells. Time is shown as (h:min) relative to the first image of the series. Scale bar represents 10 µm. e Quantitation of cell death events in 0.75 and 1.0 µM APH treated HT1080 6TG H2B-mCherry cultures (mean ± s.d., n = 3 biological replicates scoring ≥57 mitotic events per condition for 0.75 µM APH and n = 4 biological replicates scoring ≥91 mitotic events per condition for 1.0 µM APH). Source data are provided as a Source Data file
