Fig. 4
Replication stress induces WAPL-dependent cohesion fatigue. a Time course for experimentation in (b, c). b Representative images of cytogenetic chromosome spreads depicting the replication stress-induced cohesion phenotypes from HT1080 6TG cells stained with DAPI (blue), centromere (red) and telomere (green) FISH. c Quantitation of cohesion phenotypes depicted in (b) in HT1080 6TG cells treated with DMSO or APH, ± reversine (REV), colcemid or Taxol (mean ± s.e.m., n = 3 biological replicates scoring ≥ 152 chromosome spreads per condition, Fisher’s Exact Test). d Experimental time course for (e–g). e Western blots of whole cell extracts from HT1080 6TG cells treated with WAPL or control siRNA. f Representative images of cytogenetic chromosome spreads from HT1080 6TG cells treated with APH and siRNA. g Quantitation of cohesion phenotypes in HT1080 6TG cells treated with APH ± siRNA (mean ± s.e.m., n = 3 biological replicates scoring ≥ 144 chromosome spreads per condition, Fisher’s Exact Test). For all panels **p < 0.01. Scale bars represent 10 µm. Source data are provided as a Source Data file
