Fig. 5
Type 1 mitotic death is WAPL and BAX/BAK dependent. a Mitotic duration of HT1080 6TG cells treated with APH ± siRNA (three biological replicates scoring n ≥ 429 mitoses per condition are compiled in a Tukey box plot, Mann–Whitney test). b) Outcome of the mitotic events in (a) (mean ± s.e.m., n = 3 biological replicates, Fisher’s Exact Test). c Representative images of cell division generating micronuclei, or mitotic slippage, in APH treated HT1080 6TG H2B-mCherry cells. Time is shown as (h:min) relative to the first image in the series. d Mitotic outcomes in HT1080 6TG H2B-mCherry cells treated with APH ± WAPL siRNA or BAX BAK DKO (three biological replicates scoring n = 97 APH, n = 38 APH + WAPL knock down, n = 88 APH + BAX BAK DKO cells, Student t-test or Fisher’s Exact test). e Western blots of whole cell extracts from HT1080 6TG BAX BAK DKO cells treated with WAPL or control siRNA. f Mitotic duration of HT1080 6TG BAX BAK DKO cells treated with APH ± siRNA (three biological replicates scoring n ≥ 86 mitoses per condition are compiled in a Tukey box plot, Mann–Whitney test). g Quantitation of cohesion phenotypes as depicted in Fig. 4b in HT1080 6TG BAX BAK DKO cells treated with DMSO or APH (mean ± s.e.m, n = 3 biological replicates scoring ≥ 50 chromosome spreads per condition, Fisher’s Exact Test). h Timeline of the experiment shown in (i, j). i Images of DAPI stained nuclei depicting the indicated nuclear phenotypes. j Quantitation of the phenotypes shown in (i) from HT1080 6TG cells and derivatives at the 72-hour time point as indicated in (h) (n = 3 biological replicates scoring 100 nuclei per replicate, mean ± s.e.m, Fisher’s exact test). For all panels ns = not significant, **p < 0.01. Scale bars represent 10 µm. Source data are provided as a Source Data file
