Fig. 2
From: Molecular basis of microhomology-mediated end-joining by purified full-length Polθ
Substrate requirements for Polθ MMEJ of ssDNA. a–c, f, h–k Non-denaturing gels showing MMEJ reactions performed by 3 nM Polθ and 3 nM Polθ-pol on the indicated ssDNA substrates. 3′ terminal microhomology sequences are indicated (left). Bar plots showing % MMEJ (right). n = 3 ± s.d. d Bar plot showing % MMEJ by 3 nM Polθ on ssDNA of variable lengths with identical 6 bp microhomology. n = 3 ± s.d. e Non-denaturing gel showing MMEJ reactions performed by 3 nM Polθ in the presence of the indicated radio-labeled and cold ssDNA substrates. g Bar plot showing % MMEJ by 3 nM Polθ on 68–73 nt ssDNA substrates containing the indicated 3′ terminal microhomology sequences. n = 3 ± s.d. P values reported are from unpaired Student’s two-sided t-test. *, 32P. Source data are provided as a Source Data file
