Fig. 2

GluA2(R)/γ-2 single-channel recordings. a GluA2(R)/γ-2 currents from an outside-out patch containing few channels (–60 mV). Forty consecutive applications of 10 mM glutamate (gray bar) are overlaid. b Individual responses exhibiting discrete channel openings superimposed on a persistent steady-state current (upper sweeps) or exhibiting only a persistent steady-state component (lower sweeps). Note the decay of the steady-state currents on glutamate removal (gray arrows) is much slower than the closure of resolved channels (black arrow). c Histogram of channel conductance for 392 discernible openings from six patches. The histogram is fit with the sum of four Gaussian curves (dashed lines) with a common standard deviation (1.2 pS), revealing four peaks (3.5, 6.9, 10.3, and 14.1 pS)