Fig. 6

A model with access to only desensitized states predicts behavior of cross-linked GluA2(R) S729C/γ-2. a Scheme 2 is modified from Scheme 1 (Fig. 5a) and assumes that, following cross-linking, the receptor can occupy only desensitized states (excluded states are shown in gray). b Simulated responses to 10 mM glutamate (gray bar) using Scheme 1 to mimic the non-cross-linked condition and Scheme 2 to mimic the effect of cross-linking. c Representative currents at –60 mV activated by 10 mM glutamate (gray bar) from GluA2(Q) G724C/γ-2 and GluA2(R) G724C/γ-2 in DTT (black) or CuPhen (red). Note that, for both forms, currents are fully inhibited following cross-linking by 10 µM CuPhen. d Representative responses from individual patches demonstrate that following cross-linking by 10 µM CuPhen, GluA2(Q) S729C/γ-2 currents are inhibited, while GluA2(R) S729C/γ-2 currents show minimal desensitization and continue to display a large steady-state current, as predicted in a. Gray boxes (b and d) highlight the similarity of modeled currents and recorded GluA2(R) S729C/γ-2 currents