Fig. 2

miR-574-5p and -3p divergently control GC cell growth and metastasis. a The effects of miR-574-5p/-3p overexpression on GC cell (MGC-803 and HGC-27) proliferation (left), colony formation ability (middle), and early apoptosis (right). Three technical replicates from a single experiment representative of three independent experiments. b Photographs of MGC-803-engrafted tumors treated with scramble or miR-574-5p/-3p mimics at the end of the experiment (day 40), and tumor volumes as well as tumor weight are shown at the indicated days (n = 4). c Immunohistochemistry analysis of Ki-67 and caspase-3 expression in tumors from xenograft mice. d Bioluminescence imaging of mice showed GC cell metastasis in vivo and H&E staining of the lung tissues. e The relative percent of metastatic nodules formation in lung. f The survival rate of mice transplanted with MGC-803 cells treated with scramble or miR-574-5p/-3p mimics. Data are shown as means ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.