Fig. 6

Arm-imbalance of miR-574 promoted GC progression. MGC-803 cells were treated with miR-574-5p/3p mimics (5p-OE/3p-OE) and miR-574-5p/3p inhibitors (5p-KD/3p-KD) at multiple combinations to mimic the arm-imbalance. The proliferation rate (a), percentage of apoptosis (b), invasive cell number (c), and wound closure percentage (d) of MGC-803 cells of the above seven groups was analyzed. Three technical replicates from a single experiment representative of two independent experiments. e Photographs of MGC-803-engrafted tumors in the seven different groups (n = 5). f Graph representing tumor volumes. g Tumor mass of the seven groups at the end of the experiment (day 40). h Bioluminescence imaging of mice showed GC cell metastasis in vivo and the typical H&E staining of the lung tissues. i Number of metastatic nodules formation in lungs. j Representative pictures of the lungs from mice in (h). Arrows indicated nodules. Data are shown as means ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.