Fig. 3
From: Time elapsed between Zika and dengue virus infections affects antibody and T cell responses
ZIKV immunity does not exacerbate levels of pro-inflammatory cytokines. Cytokines and chemokines expression levels were determined in serum (pg/ml) by multiplex bead assay (Luminex) at baseline, 1, 2, 3, 5, 10, 15, and 30 days after DENV infection. The panel includes: a interferon alpha (IFN-α), b interleukin-6 (IL-6), c monokine induced by IFN-gamma (MIG/CXCL9), d monocyte chemoattractant protein 1 (MCP-1/CCL2), e macrophage inflammatory protein 1-beta (MIP-1β/CCL4), f IL-1 receptor antagonist (IL-1RA), g C–X–C motif chemokine 10 (CXCL10/IP-10), and h perforin. Symbols connected with lines represent mean expression levels detected of each cytokine/chemokine per cohort over time: blue squares (ZIKVPF-10mo), orange squares (ZIKVPR-2mo), and black circles (naive). Error bars indicate the standard error of the mean (SEM) for each cohort per timepoint. Cutted line marks the limit of detection for each individual cytokine/chemokine. Statistically significant differences between groups were calculated using two-way ANOVA adjusted for Tukey’s multiple comparisons test including eight families, and three comparisons per family. Significant multiplicity adjusted p-values (*< 0.05, **< 0.01, ***<0.001, ****< 0.0001) are shown colored representing the cohort against that particular point where is a statistically significant difference between groups. Source data are provided as a Source Data file
