Fig. 4
From: Time elapsed between Zika and dengue virus infections affects antibody and T cell responses
Neutralization of DENV serotypes by ZIKV-immune animals is higher in magnitude. The magnitude of the neutralizing antibody (NAb) response was determined (a) before and (b) 30 days after DENV infection by plaque reduction neutralization test (PRNT) against all DENV serotypes. c–f The durability of the neutralizing response was assessed measuring NAb titers up to 90 dpi against all DENV serotypes. Symbols connected with full lines indicate mean levels of NAb titers detected per cohort over time: blue squares (ZIKVPF-10mo), orange squares (ZIKVPR-2mo), and black circles (naive). Error bars represent the standard error of the mean (SEM). PRNT60: NAb titer capable of reduce 60% or more of DENV serotypes plaque-forming units (pfu) compared with the mock (control of virus without serum). A PRNT60 1:20 titer was considered positive, and < 1:20 as a negative Neut titer. Dotted line mark < 1:20 for the negative results. Non-neutralizing titers (< 1:20) were assigned with one-half of the limit of detection for graphs visualization (1:10). Statistically significant differences between groups were calculated using two-way ANOVA adjusted for Tukey’s multiple comparisons test including four and six families for heterologous serotypes and DENV-2, respectively, and three comparisons per family. Significant multiplicity adjusted p-values (*< 0.05, **< 0.01, ***< 0.001, ****< 0.0001) are shown. Blue and orange asterisks represent significant difference between the corresponded ZIKV immune groups and naive group, and gray asterisks indicate a significant difference between ZIKV immune groups. Source data are provided as a Source Data file
