Fig. 8
Single-cell RNAseq reveals distinct cell populations within P and D organoids. a Transcriptomic clusters in P (n = 6826 cells) and D (n = 4896 cells) organoids visualized using uniform manifold approximation and projection (uMAP) plots, colored according to cluster. b Proportion of cells from P and D organoids contributing to each of the clusters shown in panel a. c Heatmap depicting expression of selected cluster markers identified by differential expression analysis (Wilcoxon rank-sum test). d Dot plot depicting the expression of significantly differentially expressed genes from the Wnt and Notch signaling pathways in P and D organoids, according to cell clusters. e UMAP plots visualizing integrated analysis of cells from P and D organoids as a joint plot (left, n = 11722) or separate plots (P, center; D, right). For P organoids: B (Basal), BP (Basal-Proliferative), IL (Intermediate-Low) and IH (Intermediate-High); for D organoids: B (Basal), I (Intermediate), and L (Luminal). Source data are provided as a Source Data file
