Fig. 1
From: CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity
Generation and characterization of CAR-T cells. a Vector maps of tested CAR designs. b Membrane-bound CAR expression. Forty-eight hours after retroviral transduction, the expression of CAR on human T cells was detected by staining with anti-MYC antibody, followed by flow cytometry analysis. T cells without transduction were used as a negative control. The histograms shown in black correspond to the isotype controls, whereas the red histograms indicate the positive fluorescence. c Killing activity of CAR-T cells in response to tumour cells. The cytotoxic activity of CAR-T and control T cells against cancer cell lines was assessed by a 51Cr-release assay at the indicated effector-to-target (E:T) ratios. Results shown represent three (b) independent experiments. Data are means ± s.d. of five (c) independent biological replicates. P values are from a two-way ANOVA followed by the Bonferroni post-test (c). Source data (c) are provided as a Source Data file
