Fig. 3
From: CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity
Characterization of CAR exosomes derived from effector CAR-T cells. a Schematic representation of the enrichment of CAR-containing exosomes from T cells with repeated antigen stimulation. b Size distribution of CAR exosomes as measured by NTA, peaking at an 85-nm diameter. c Transmission electron micrographs of CAR exosomes. The samples were negatively stained with uranyl acetate. Scale bars = 100 nm. d Immunoblots for CAR expression in exosomes compared with cell markers for endoplasmic reticulum (calregulin), Golgi (Golgi 58 K), mitochondrial (prohibitin), or nuclear (nucleoporin p62) markers. e Flow cytometry analyses of CAR exosomes linked to latex beads (4-mm diameter) or CAR-T cells and stained with the indicated primary Abs. The histograms shown in black correspond to the isotype controls of the respective Abs, whereas the red histograms indicate positive fluorescence. Results shown represent three (b–e) independent experiments. Source data (d) are provided as a Source Data file
