Fig. 4
From: CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity
Cytolytic activity of CAR exosomes in vitro. a Flow cytometry analyses of CAR exosomes linked to latex beads (4-mm diameter) or CAR-T cells stained with the indicated primary Abs. The histograms shown in black correspond to the isotype controls of the respective Abs, whereas the red histograms indicate positive fluorescence. b Immunoblots for perforin and granzyme B expression in CAR exosomes and CAR-T cells. c Killing activity of CAR exosomes in response to tumour cells. The cytotoxic activity of CAR exosomes and control T cells against cancer cell lines was assessed by the 51Cr-release assay at the indicated concentration. d Confocal microscopy analysis of MCF-7 EGFR cells (up) and MCF-7 HER2 cells (down) after incubation with NHS-Rhodamine (Rho)-labelled CAR-EXO-CTX for 2 h. The experiments were repeated independently three times with similar results. Scale bars = 10 μm. Results shown represent three (a, b, d) independent experiments. Data are the means ± s.d. of three (c) independent biological replicates. P values were from a nonparametric t test (c). Source data (b, c) are provided as a Source Data file
