Fig. 1

High rates of inter-homolog recombination during concerted chromosome loss (CCL). a Schematic showing the construction and screening of a genetically marked tetraploid strain for monitoring chromosome loss and recombination. b Frequency of different ploidy states in 2-DOG^R (gal1⁻) progeny derived from tetraploid cells by CCL. Error bars signify standard deviation. n = 3 biologically independent experiments. c Frequency of 2-DOG^R colonies following growth of the genetically marked tetraploid strain on solid agar under standard conditions (green; YPD, 30 °C) or under conditions that promote CCL (white; PRE-SPO medium, 37 °C) for 7 days. n = 4 biologically independent experiments. d Example images of selection plates indicating growth of progeny on selective media for different markers. e Recombination frequencies in HIS1-SAT1 and SAT1-URA3 intervals following growth on standard medium (green) or CCL-inducing medium (white) for 7 days. SAT1 encodes resistance to nourseothricin (NAT^R). Boxplots are presented as the 75th to 25th percentile with the thick line denoting the median. Whiskers indicate the largest and smallest values within 1.5 × of the interquartile range. f C. albicans tetraploid cells expressing the Gam-GFP construct following 24 h growth on PRE-SPO or YPD medium (CCL-inducing or standard growth conditions, respectively). Scale bar, 5 μm. Error bars indicate standard deviation