Fig. 4
From: A ‘parameiosis’ drives depolyploidization and homologous recombination in Candida albicans
Spo11, but not Rec8, promotes recombination in mitotically dividing C. albicans cells. a–d Genetically marked diploid and tetraploid C. albicans strains were serially passaged in YPD medium at 30 °C or 37 °C (cells diluted 1:100 every 24 h), and evaluated for loss of the GAL1 marker (2-DOGR frequency) and for the frequency of recombination in the HIS1-URA3 interval. a Frequency of 2-DOGR colonies in diploid/tetraploid wildtype and SPO11 mutant strains. n = 4, 5, 6, and 6 biologically independent experiments for 30 oC diploid, 37 oC diploid, 30 oC tetraploid, and 37 oC tetraploid, respectively. b Frequency of recombination between HIS1 and URA3 on Chr1 in diploid/tetraploid wildtype and SPO11 mutant strains. n = 4, 4, 5, and 5 biologically independent experiments for 30 oC diploid, 37 oC diploid, 30 oC tetraploid, and 37 oC tetraploid, respectively. c Frequency of 2-DOGR colonies in wildtype or REC8 mutant tetraploid strains passaged at 30 oC. n = 4 biologically independent experiments. d Analysis of recombination in the HIS1-URA3 interval for wildtype or REC8 mutant tetraploid strains passaged at 30 oC. n = 4 biologically independent experiments. e Tenfold spot dilution assays performed for wildtype, Δspo11, and SPO11-complemented tetraploid cells grown on YPD medium in the presence or absence of 0.01% methyl methanesulfonate (MMS) and imaged after 2 days at 37 oC. * denotes p < 0.05. ** denotes p < 0.01 as calculated by two-sample t (chromosome loss) or Wilcoxon (recombination frequency) tests. Boxplots are presented as the 25th to 75th percentile with the thick line denoting the median. Whiskers indicate the largest and smallest values within 1.5 × of the interquartile range. White, orange, light orange, and gray denote WT, Δspo11, Δ+SPO11(YF), and Δ+SPO11(WT), respectively. White, purple, and gray denote WT, Δrec8, and Δ+REC8(WT), respectively
