Fig. 7
rdr6-11 partially rescues the fry1 phenotypes. a Genomic classification of 21-nt AGO1-associated sRNAs. In WT, miRNAs and ta-siRNAs constitute the majority of AGO1-associated 21-nt sRNAs. In fry1-6, there is a drastic increase in rRNA-derived siRNAs, consistent with the total sRNA composition. The rdr6 mutation results in a partial removal of risiRNAs and a concomitant partial restoration of miRNAs. The Y axis shows the cumulative RPM values for sRNAs corresponding to different genomic features. b The AGO1 loading efficiency of the 20 most abundant miRNAs in WT. The loading efficiency is represented by the ratio of RPM in immunoprecipitated samples to that in input. The efficiencies in WT and fry1-6 are significantly different based on a paired Wilcoxon test (P value = 0.001718). The efficiencies are recovered by the rdr6 mutation (fry1-6 rdr6-11 vs. fry1-6: P value = 0.003654; fry1-6 rdr6-11 vs. WT: P value = 0.114). c, d miRNA accumulation in rdr and dcl mutants. miR166 and miR398 are downregulated in fry1-6, and all three analyzed double mutants show slightly higher abundance of the miRNAs than fry1-6 (c). However, the increased abundance of miR168 in fry1-6 was still present in the double mutants (d). The internal control U6 snRNA was used to determine the relative miRNA levels. e Partial rescue of the fry1 phenotypes by rdr and dcl mutations. Plants shown are at 22 days after germination. rdr6-11 can partially rescue the mutant phenotypes of fry1-6. Meanwhile, DCL4 is necessary for the survival of fry1 mutants, probably due to the enhanced activity of DCL2 in dcl4-2. This was supported by the fry1-6 dcl2-1 dcl4-2 triple mutant. The restoration of leaf shape by rdr6-11 is shown by the enlarged leaves in the insets. Source data are provided as a Source Data file
