Fig. 7
Miro links the microtubule transport pathway to the MICOS complex through TRAK. a Quantification of the distribution of OMM and IMM components upon TRAK1/KIF5C overexpression in micropatterned substrates. WT and MiroDKO cells expressing the Tom70(1–70)GFP together with the mitochondrial motor machinery TRAK1/KIF5C were grown in “Y”-shaped micropatterns to produce triangular cells. Cells were immunostained for endogenous expression of an OMM marker (Tom40: cyan) and an IMM marker (ATP5α: red), (scale bar: 10 μm; insets: 5 μm). b Cell representations of the relative accumulations of the OMM marker Tom40 (subtraction of the ATP5α signal from the Tom40 signal; upper row) and the IMM marker ATP5α (subtraction of the Tom40 signal from the ATP5α signal, bottom row) (scale bar: 10 μm; insets: 5 μm). c Projections of all the 76 cell tips that contained the mitochondria and generation of mitochondrial probability maps. In WT cells, both OMM and IMM markers are similarly distributed, while in the Miro DKO cells the OMM marker is preferentially accumulated in the most distal regions compared with the IMM which accumulates in more proximal regions (scale bar: 5 μm). d Projections from the 76 subtracted images (for each genotype) were generated as in (b) (scale bar: 5 μm). e Mitochondrial probability map to quantify the ratio between the normalized signals of OMM (Tom40) and IMM (ATP5α) components as a function of the distance from the center of the cell (see Supplementary experimental procedures for details). All experiments were performed three independent times. Quantification and statistics in (e) were performed with 32 cells for each genotype (n = cells; Student's t test was performed at each distance point). Error bars represent ± SEM. Significance: *p < 0.05; **p < 0.01; and ***p < 0.001
