Fig. 2
From: Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis
Frequency and kinetics of dyn2 recruitment events in dynPRD mutants. a TIRF images of living SKMEL cells expressing dyn2-GFP in both endogenous loci (left) or dynamin TKO MEF cells transfected with the indicated dyn2-GFP mutants (right). Scale bar 10 µm. b Average fluorescence outside the dyn2-GFP clusters for the various dyn2-GFP mutants. The number of cells recorded is indicated at the top of the graph. c dyn2-GFP recruitment event frequencies for the same cells as in (b). d Average amplitudes of peak dyn2-GFP events for the same cells as in (b), except for cells transfected with dyn2-GFP-ΔCter (n.a.). e Normalized average fluorescence of all the events detected, aligned to their peak fluorescence. The total number of events averaged was 36070 for dyn2-GFPen cells and 18678, 127, 5916 and 6116 for TKO cells transfected with dyn2-GFP-WT, dyn2-GFP-ΔCter, dyn2-GFP-Bmut and dyn2-GFP-ACmut, respectively. f TIRF images of portions of dynamin TKO cells co-transfected with amphiphysin-mCherry and the indicated dyn2-GFP mutants. Scale bar 2 µm. g Frequencies (top) and peak amplitudes (bottom) of amph-mCherry recruitment events recorded in dynamin TKO cells co-transfected with the indicated dyn2-GFP mutants. N = 5–7 cells for each condition, no significant difference with WT in any condition
