Fig. 1

Reduction of peripheral CD8⁺, but not CD4⁺, T cells in Stat5^fl/−CD8Cre mice. a Deletion of Stat5 in YFP⁺ CD8 T cells. CD4⁺ and YFP⁺CD8⁺ T cells from Stat5^+/+CD8Cre/YFP (+/+) and Stat5^fl/−CD8Cre/YFP (fl/−) mice were subjected to Western blot analysis with the indicated antibodies. b CD8Cre-mediated Stat5 deletion in CD4⁻CD8⁺, but not CD4⁺CD8⁺ or CD4⁺CD8⁻, thymocytes. Thymocytes from Stat5^+/−CD8Cre/YFP (Stat5^+/−CD8Cre) and Stat5^fl/−CD8Cre/YFP (Stat5^fl/−CD8Cre) mice were stained with anti-CD4 and anti-CD8 antibodies. Numbers indicate percentages of YFP⁺ cells in the gated CD8⁺ population. c Normal development of thymocytes in Stat5^fl/−CD8Cre/YFP mice. Numbers indicate percentages of CD4⁻CD8⁻, CD4⁺CD8⁺, CD4⁺CD8− or CD4⁻CD8⁺ thymocytes in the gated thymic lymphoid population. d CD8Cre-mediated Stat5 deletion in CD8⁺, but not CD4⁺, splenic T cells. Splenocytes were stained with anti-CD4 and anti-CD8 antibodies. Numbers indicate percentages of YFP⁺ cells in the gated CD8⁺ population. e Marked reduction of splenic CD8⁺ T cells in Stat5^fl/−CD8Cre/YFP mice. Numbers indicate percentages of CD4⁺ and CD8⁺ T cells in the gated splenic lymphoid population. f Dot plots show percentages of CD4⁺ and CD8⁺ T cells (left) and the numbers of CD4⁺ and CD8⁺ T cells (right) in the spleen. Each dot represents an individual mouse and horizontal bars indicate mean values. Mean ± SD is shown. P-values were calculated with the unpaired two-tailed Student’s t-test. Data shown are representative of 2 (a) independent experiments or obtained from 6 (b, c) or 8 (d–f) Stat5^+/−CD8Cre/YFP and 6 (b, c) or 9 (d–f) Stat5^fl/−CD8Cre/YFP mice. Source data are available as a source data file