Fig. 2
From: CXCR5+PD-1+ follicular helper CD8 T cells control B cell tolerance
Increased autoantibody production in Stat5fl/−CD8Cre mice. a Normal numbers of splenic B cells in Stat5fl/−CD8Cre mice. Splenocytes from Stat5+/−CD8Cre and Stat5fl/−CD8Cre mice were stained with anti-B220. Dot plots show percentages of B220+ B cells (left) and numbers of total splenocytes and B220+ B cells (right) in the spleen. Each dot represents an individual mouse and horizontal bars indicate mean values. Mean ± SD is shown. b Normal serum Ig levels in Stat5fl/−CD8Cre mice. The serum levels of the Ig isotypes in 2-month-old Stat5+/−CD8Cre and Stat5fl/−CD8Cre mice were determined. Each dot represents an individual mouse and horizontal bars indicate mean values. Mean ± SD is shown. c Increased IgG autoantibody production in Stat5fl/−CD8Cre mice. The sera from 2-month-old mice were screened by the autoantigen array. d Self-tolerance breakdown in Stat5fl/−CD8CreIgHELsHEL mice. Dot plots show the serum levels of HEL-specific IgM in 2-month-old Stat5+/−CD8CreIgHELsHEL and Stat5fl/−CD8CreIgHELsHEL mice. Each dot represents an individual mouse and horizontal bars indicate mean values. Mean ± SD is shown. P-values were calculated with the unpaired two-tailed Student’s t-test. Data shown are obtained from 8 (a), 11 (b) or 7 (c) Stat5+/−CD8Cre/YFP, 9 (a), 13 (b) or 8 (c) Stat5fl/−CD8Cre/ YFP mice, 8 (d) Stat5+/−CD8CreIgHELsHEL and 13 (d) Stat5fl/−CD8CreIgHELsHEL mice. Source data are available as a source data file
